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What is HPLC ? A Beginner’s Guide to High-Performance Liquid Chromatography

What is HPLC ? A Simple Introduction for Beginners High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique used to separate, identify, and quantify the components in a mixture. It is widely applied in pharmaceutical, environmental, food, and chemical industries for quality control and research. In the pharmaceutical industry, HPLC is essential for ensuring the safety and efficacy of drugs by analyzing the purity, potency, and stability of active pharmaceutical ingredients (APIs) and formulations. Why is HPLC Important? Even minor variations in drug composition can lead to serious health risks. HPLC provides precise and reliable data that help manufacturers comply with regulatory standards and deliver safe medications to patients. It is also used for: Detecting impurities and degradation products Quantifying drug release rates in formulations Monitoring stability under different environmental conditions Basic Principle: HPLC separates c...
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Types of HPLC – Explained with Examples

Types of HPLC – Explained with Examples 🔬 High-Performance Liquid Chromatography ( HPLC ) has different types depending on how the separation is done. Each type is designed to handle specific compounds and sample properties . In this post, you’ll learn the main types of HPLC used in the pharmaceutical industry and how they work. 1. Reverse-Phase HPLC (RP-HPLC) 🔄 Reverse-phase HPLC is the most commonly used type in pharma labs. In this method, the stationary phase is non-polar (hydrophobic), and the mobile phase is polar (water-based solvents). Used for: Non-polar or moderately polar compounds Example: Testing drug substances like paracetamol, ibuprofen, or vitamins 2. Normal-Phase HPLC (NP-HPLC) ⚖️ This method uses a polar stationary phase (like silica) and a non-polar mobile phase (like hexane). It separates polar compounds better than RP-HPLC. Used for: Separation of isomers, sugars, amino acids Example: Identification of sm...
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HPLC Columns Explained: Types, How to Choose & Maintain

Understanding HPLC Columns: Types, Selection & Maintenance 🔬 The HPLC column is the most critical part of the chromatography system. It holds the stationary phase and is responsible for separating the compounds in your sample. The choice of column directly impacts the quality, speed, and reproducibility of your analysis. What is an HPLC Column? An HPLC column is a stainless steel tube packed with tiny particles called the stationary phase . When the liquid mobile phase pushes the sample through the column, interactions between the analytes and stationary phase cause them to separate and elute at different times. Types of HPLC Columns Columns are categorized based on the type of stationary phase they contain: Reverse-Phase Columns (RP): These are the most widely used columns in pharmaceutical analysis. They use non-polar stationary phases, commonly octadecyl (C18) or octyl (C8) bonded silica. Reverse-phase columns are excellent for separating non-polar to moderately ...
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